I'm on holiday this week so this is an old post that appeared on my previous blog "Life of a Lab Rat" on July 1st 2010.

Prokaryotes are by far the most successful superkingdom in terms of both biochemical diversity and the variety of environments conquered. Bacteria can be found living in all kinds of adverse conditions; from high alkaline lakes, to below freezing temperature, to hot volcanic vents which in some cases can reach temperatures close to the boiling point of water.

Thermotoga is a small genus of bacteria that contains some of the most hyperthermophilic species known, some able to survive at 90°C although most prefer the cooler temperatures of 70-80°C. It’s called “thermotoga” because it lives at high temperatures (thermo) and contains a characteristic outer cell membrane known as the ‘toga’.

One of the most interesting things about bacteria that survive in very high temperatures is their enzymes. Most normal enzymes will break down and denature at very high temperatures, so bacteria like thermotoga will usually have their own special set. These enzymes are usually of great interest to people carrying out industrial processes, which all take place at higher temperatures. The most important enzyme in the PCR protocol (Taq polymerase, which synthesises DNA at temperatures of up to 80°C) was isolated from a marine thermophilic archaea.

Another exciting thing about Thermotoga is that it has the ability to produce hydrogen. In the lab it uses carbohydrates from yeast extract or peptone to form sugars which are oxidised to carbon dioxide or acetic acid. If protons are used as the electron acceptor they get reduced to hydrogen as the carbon dioxide is formed. The yield of hydrogen from glucose is very high and in many cases can approach the theoretical maximum yield (4 mole hydrogen from one mole of glucose). This represents almost twice the amount that can be obtained from other bacterial hydrogen producers. Because this process is taking place at high temperatures, the enzymes involved in the process could potentially be used inside high temperature reactors.

Thermotoga neopolitana has the potential to produce one of the highest hydrogen yields of all as it is able to respire microaerobically. This means that it would be theoretically possible for the cells to aerobically oxidise a small amount of the glucose, generating enough energy to ensure that all the remaining glucose is fully oxidised by hydrogen-generating pathways. As yet, the metabolic pathways for oxygen use within these organisms have not been identified, but it does look as if hydrogen generation depends almost exclusively on anaerobic processes.

As the majority of the research into hydrogen production of Thermotoga has focussed on yield issues of productivity, stability and required substrates will all need to be addressed in order for this process to be fully understood and possibly implemented in an industrial setting. There are also issues with biomass concentration which is restricted, possibly by free-flowing biofilms (and possibly due to the toga-like nature of the surrounding cell-wall capsules). However even if large scale hydrogen production proves to be unfeasible with these microorganisms, studies of the enzymatic processes used to produce hydrogen at high temperatures may have applications above and beyond using the entire bacteria for hydrogen production.


Van Ooteghem, S., Beer, S., & Yue, P. (2002). Hydrogen Production by the Thermophilic Bacterium Thermotoga neapolitana Applied Biochemistry and Biotechnology, 98-100 (1-9), 177-190 DOI: 10.1385/ABAB:98-100:1-9:177